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- Clone
- MI15 (See other available formats)
- Workshop
- HCDM listed
- Other Names
- B-B4
- Isotype
- Mouse IgG1, κ
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Compare all formats See GMP FITC spectral data
Cat # | Size | Price | Quantity Check Availability | Save | ||
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260398 | 100 tests | £161 |
Description
CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells, but not on mature circulating B-lymphocytes. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial cells, and neural cells.
Product Details
Quality Statement This BioLegendâs GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.
Product Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A mixture of U266 and XG-1 human myeloma cell lines.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- 200 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.4
Additional reported applications for the relevant formats include: immunofluorescent staining1, Western blotting2, immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections3, and spatial biology (IBEX)5,6.
- Application References
(PubMed link indicates BioLegend citation)
-
- Costes V, et al. 1999. Hum. Pathol. 30:1405. (IF)
- Gattei V, et al. 1999. Br. J. Haematol. 104:152. (WB)
- Bologna-Molina R, et al. 2008. Oral Oncol. 44:805. (IHC)
- Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482.
- Radtke AJ,et al. 2020.Proc Natl Acad Sci USA. 117:33455-33465. (SB)PubMed
- Radtke AJ,et al. 2022.Nat Protoc. 17:378-401. (SB)PubMed
- Disclaimer
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Antigen Details
- Structure
- 100-200 kD type I integral transmembrane glycoprotein
- Distribution
-
Plasma cells, pre-B cells, epithelial cells, endothelial cells
- Function
- Adhesion, controls cell morphology, regulates cell growth
- Ligand/Receptor
- FGFb, collagen, fibronectin
- Cell Type
- B cells, Endothelial cells, Epithelial cells, Plasma cells
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. Wiley-Liss A John Wiley & Sons Inc, Publication. - Gene ID
- 6382 View all products for this Gene ID
- UniProt
- View information about CD138 on UniProt.org
Documentation
- Technical Data Sheet (pdf)
- Certificate of Analysis
- Safety Data Sheet
Reviews
Review This Product
Related Protocols
- Cell Surface Flow Cytometry Staining Protocol
Related Products
Description | Clone | Applications |
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Pathways
- B cell Development and Activation
Pages
- Quality Control
- Multicolor Staining Guide
- Fluorescence Spectra Analyzer
- CD Molecule Chart
- Fluorophore Brightness Index
- Custom Panel Construction
- Fluorophore Equivalency Tool
Related FAQs
There are no FAQs for this product.
Other Formats
View All CD138 Reagents Request Custom Conjugation
Description | Clone | Applications |
---|---|---|
PE anti-human CD138 (Syndecan-1) | MI15 | FC,SB |
Purified anti-human CD138 (Syndecan-1) | MI15 | FC,ICC,WB,IHC-P,SB |
APC anti-human CD138 (Syndecan-1) | MI15 | FC |
FITC anti-human CD138 (Syndecan-1) | MI15 | FC,SB |
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1) | MI15 | FC |
Alexa Fluor® 700 anti-human CD138 (Syndecan-1) | MI15 | FC |
PE/Cyanine7 anti-human CD138 (Syndecan-1) | MI15 | FC |
Brilliant Violet 421™ anti-human CD138 (Syndecan-1) | MI15 | FC |
Brilliant Violet 510™ anti-human CD138 (Syndecan-1) | MI15 | FC |
Brilliant Violet 605™ anti-human CD138 (Syndecan-1) | MI15 | FC |
Brilliant Violet 711™ anti-human CD138 (Syndecan-1) | MI15 | FC |
Alexa Fluor® 647 anti-human CD138 (Syndecan-1) | MI15 | FC,SB |
Alexa Fluor® 594 anti-human CD138 (Syndecan-1) | MI15 | ICC |
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1) | MI15 | FC |
APC/Cyanine7 anti-human CD138 (Syndecan-1) | MI15 | FC |
Pacific Blue™ anti-human CD138 (Syndecan-1) | MI15 | FC |
TotalSeq™-A0055 anti-human CD138 (Syndecan-1) | MI15 | PG |
Brilliant Violet 785™ anti-human CD138 (Syndecan-1) | MI15 | FC |
Biotin anti-human CD138 (Syndecan-1) | MI15 | FC |
TotalSeq™-C0055 anti-human CD138 (Syndecan-1) | MI15 | PG |
APC/Fire™ 750 anti-human CD138 (Syndecan-1) | MI15 | FC |
TotalSeq™-B0055 anti-human CD138 (Syndecan-1) | MI15 | PG |
PE/Cyanine5 anti-human CD138 (Syndecan-1) | MI15 | FC |
TotalSeq™-D0055 anti-human CD138 (Syndecan-1) | MI15 | PG |
PE/Fire™ 640 anti-human CD138 (Syndecan-1) | MI15 | FC |
Spark Violet™ 500 anti-human CD138 (Syndecan-1) | MI15 | FC |
FITC anti-human CD138 | MI15 | FC |
PE/Fire™ 700 anti-human CD138 (Syndecan-1) | MI15 | FC |
Spark Violet™ 423 anti-human CD138 (Syndecan-1) | MI15 | FC |
Spark Red™ 718 anti-human CD138 (Syndecan-1) (Flexi-Fluor™) | MI15 | FC |
GMP FITC anti-human CD138 (Syndecan-1) | MI15 | FC |
Brilliant Violet 650™ anti-human CD138 (Syndecan-1) | MI15 | FC |
Certificate of Analysis
For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.
This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for userâs intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.
BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.
8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... Confocal image of human lymph node sample acquired using the... Confocal image of human liver sample acquired using the IBEX... Confocal image of human jejunum sample acquired using the IB... -
Purified anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with purified CD138... SeqIF⢠(sequential immunofluorescence) staining on COMET⢠of... -
APC anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
FITC anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (Syndeca... -
Alexa Fluor® 700 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
PE/Cyanine7 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Brilliant Violet 421™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Brilliant Violet 510™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Brilliant Violet 605™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Brilliant Violet 711™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Alexa Fluor® 647 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... Confocal image of human skin sample acquired using the IBEX ... Confocal image of human metastatic lymph node sample acquire... -
Alexa Fluor® 594 anti-human CD138 (Syndecan-1)
U266B1 cells were fixed with 2% paraformaldehyde (PFA) for t... -
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
APC/Cyanine7 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Pacific Blue™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with Pacific Blue⢠... -
TotalSeq™-A0055 anti-human CD138 (Syndecan-1)
-
Brilliant Violet 785™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Biotin anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (Syndeca... -
TotalSeq™-C0055 anti-human CD138 (Syndecan-1)
-
APC/Fire™ 750 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (Syndeca... -
TotalSeq™-B0055 anti-human CD138 (Syndecan-1)
-
PE/Cyanine5 anti-human CD138 (Syndecan-1)
U266 (human myeloma cell line) cells were stained with anti-... -
TotalSeq™-D0055 anti-human CD138 (Syndecan-1)
-
PE/Fire™ 640 anti-human CD138 (Syndecan-1)
U266 cells were stained with anti-human CD138 (Syndecan-1) (... -
Spark Violet™ 500 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with anti-human CD1... -
FITC anti-human CD138
Typical results from human myeloma cell line U266 stained ei... -
PE/Fire™ 700 anti-human CD138 (Syndecan-1)
U266 cells were stained with anti-human CD138 (Syndecan-1) (... -
Spark Violet™ 423 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with anti-human CD1... -
Spark Red™ 718 anti-human CD138 (Syndecan-1) (Flexi-Fluor™)
-
GMP FITC anti-human CD138 (Syndecan-1)
Typical results from human myeloma cell line U266 stained ei... -
Brilliant Violet 650™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with anti-human CD1...